CHEK2 antibody (pThr68)

Catalog No. ABIN7250719

The Rabbit Polyclonal anti-CHEK2 antibody has been validated for WB, ELISA, IF and IHC (p). It is suitable to detect CHEK2 in samples from Human, Mouse and Rat.

Quick Overview for CHEK2 antibody (pThr68) (ABIN7250719)

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHEK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CHEK2 Antibody

Binding Specificity: pThr68

Characteristics: Phosphorylated antibody

Purification: Affinity purification

Immunogen: Synthesized peptide derived from human Chk2 around the phosphorylation site of Thr68

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:50-1:200, ELISA 1:10000-1:20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 0.5 % BSA and 50 % glycerol, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for CHEK2

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Alternative Name: CHEK2

Background: Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.

Molecular Weight:

Observed_MW: 61 kDa

Calculated_MW: 61 kDa

UniProt: O96017

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle